Patricia Simner│Applying Nanopore Sequencing and Real Time Antimicrobial Resistance Gene Analysis to
More than 700,000 people around the world die from antibiotic-resistant infections annually, translating to 1,900 deaths per day. One of the most significant antibiotic resistance threats is carbapenem-resistant Gram-negative organisms (CRO). This presentation will review methods for which Nanopore sequencing and real-time antimicrobial resistance gene analysis can help to address the threat of CRO as a diagnostic tool in the Medical Microbiology Laboratory. Applications reviewed will include detection of gastrointestinal colonization with CRO using metagenomics next-generation sequencing and real-time resistance gene analysis of whole genome sequencing data from carbapenem-resistant organisms to predict therapy. These applications of nanopore sequencing in the hospital setting has the potential to impact infection control and antimicrobial stewardship initiatives.
Flongle, GridION, MinION, MinIT, PromethION, and VolTRAX are currently for research use only.